Antibodies

Mouse monoclonal antibody to HIV-1 Tat

A2-821-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Tat
Immunogen HIV-1 Tat
Immunogen Description Recombinant Tat protein of HIV-1 HXB2 (purified from E. coli)
Clonality Mouse monoclonal
Clone 1
Class IgG2b
Reactivity Reacts with HIV-1 Tat of HXB2, IIIB, HAN strains. This antibody recognizes Tat protein consensus sequences of HIV-1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). Mapped to amino acids 2-9 EPVDPRLE ­- B subtype a.a. consensus 
Application ELISA, WB, IHC, RIP, ChIP
Protocol ELISA 0.02 to 0.05 µg/ml, WB 1 to 2 µg/ml.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
References

Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn (1994) Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.

Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn (1995) Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn (1997) B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51: 480-7.

Henderson WW, Ruhl R, Lewis P, Bentley M, Nelson JA and Moses AV. (2004) Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78: 4408-20.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren (2001) Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

Buy now
€299 / 100µg













Figure 1.  Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone 1). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml  Lane 2. Protein size marker



Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone 1). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA