Mouse monoclonal antibody to HIV-1 Tat
|Name||Mouse monoclonal antibody to HIV-1 Tat
|Immunogen Description||Recombinant Tat protein of HIV-1 HXB2 (purified from E. coli)|
|Reactivity||Reacts with HIV-1 Tat of HXB2, IIIB, HAN strains. This antibody recognizes Tat protein consensus sequences of HIV-1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). Mapped to amino acids 2-9 EPVDPRLE - B subtype a.a. consensus|
|Application||ELISA, WB, IHC, RIP, ChIP|
|Protocol||ELISA 0.02 to 0.05 µg/ml, WB 1 to 2 µg/ml.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
|Purification||Protein G purification
|Buffer||PBS pH 7.4, with 0.1% sodium azide|
|Unit Size||100 µg|
|Related Products||HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies|
|Shipping||This product is shipped in non-frozen liquid form in ambient conditions|
|Storage||Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.|
Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn (1994) Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.
Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn (1995) Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.
Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn (1997) B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51: 480-7.
Henderson WW, Ruhl R, Lewis P, Bentley M, Nelson JA and Moses AV. (2004) Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78: 4408-20.Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren (2001) Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.
This product is for research use only
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Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone 1). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml Lane 2. Protein size marker