Antibodies

Mouse monoclonal antibody to HIV-1 Rev

A2-832-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Rev
Immunogen HIV-1 Rev
Immunogen Description Recombinant Rev protein of HIV-1 BRV (purified from E. coli)
Clonality Mouse monoclonal
Clone 10
Class IgG1
Reactivity Reacts with HIV-1 Rev of subtype B. Mapped to amino acids 34-40  (TRGARRN – subtype B a.a. consensus)
Application ELISA, WB, IF, IP, IHC
Protocol ELISA 0.25 - 0.5 µg/ml; WB 2 µg/ml. 
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References

Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn (1994) Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.

Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn (1995) Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn (1997) B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51: 480-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren (2001) Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1:  Western Blot testing of HIV-1 recombinant Rev protein using HIV-1 Rev antibody, clone 10. Line 1. Recombinant HIV-1 Rev (B subtype) Line 2. Protein size marker