Mouse monoclonal antibody to HIV-1 Nef
A2-811-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 Nef |
| Immunogen | HIV-1 Nef |
| Immunogen Description | Recombinant Nef protein of HIV-1 B-subtype consensus (purified from E. coli) |
| Alternative Names | 3’ORF |
| Clonality | Mouse monoclonal |
| Clone | N5 |
| Class | IgG1 |
| Reactivity | This antibody recognizes HIV-1 subtypes A, B and C. Mapped to amino acids 62-68 (EEEVGFP – B subtype consensus) |
| Application | ELISA, WB |
| Protocol | ELISA 0.02 to 0.1 µg/ml; WB 2 µg/ml. Monoclonal antibody working titer has to be
established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| References |
Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7. Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61. |
| Limitations |
This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure 1: Western Blot testing of HIV-1 recombinant Nef protein using HIV-1 Nef antibody, clone N5 (01-011). Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker