Antibodies

Mouse monoclonal antibody to HIV-1 Nef

A2-811-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Nef
Immunogen HIV-1 Nef
Immunogen Description Recombinant Nef protein of HIV-1 B-subtype consensus (purified from E. coli)
Alternative Names 3’ORF
Clonality Mouse monoclonal
Clone N5
Class IgG1
Reactivity This antibody recognizes HIV-1 subtypes A, B and C. Mapped to amino acids 62-68 (EEEVGFP – B subtype consensus)
Application ELISA, WB
Protocol ELISA 0.02 to 0.1 µg/ml; WB 2 µg/ml.  
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1:
 Western Blot testing of HIV-1 recombinant Nef protein using HIV-1 Nef antibody, clone N5 (01-011). Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker