Mouse monoclonal antibody to HIV-1 Nef
A2-804-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 Nef |
| Immunogen | HIV-1 Nef |
| Immunogen Description |
Recombinant Nef protein of HIV-1 B subtype consensus (purified from E. coli) |
| Uniprot ID | - |
| Alternative Names | 3’ORF |
| Clonality | Mouse monoclonal |
| Clone | 3E6 |
| Class | IgG1 |
| Reactivity |
Reacts with HIV-1 Nef of subtypes A, B and C; BRU, IIIB, LAV, BH10 strains. Mapped to amino acids 163-173 (SLLHPMSLHGM – B subtypes consensus) |
| Application | ELISA, WB, ICC, RIP |
| Protocol |
ELISA 0.033 to 0.1 µg/ml; WB: 1 µg/ml Monoclonal antibody working titer has to be established
practically for each particular antigen and assay format |
| Purification |
Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide. |
| Unit Size |
100 µg |
| Related Products |
HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage |
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | - |
| References |
Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34. Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994. Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481 Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008. Fiala, A.M., R.H. Rhodes, P. Shapshak, I. Nagano, O. Martinez-Maza, A. Diagne, G. Baldwin, and M. Graves. 1996. Regulation of HIV-1 infection in astrocytes: Expression of NEF, TNF-alpha and IL-6 is enhanced in coculture of astrocytes with macrophages. J.Neurovirology, 2, No. 3: 158-166. PMID: 8799208 Koedel, U., Kohleisen B, Sporer B, Lahrtz F, Ovod V, Fontana A, Erfle V, Pfister HW. 1999. HIV type 1 Nef protein is a viral factor for leukocyte recruitment into the central nervous system. J Immunol. 1;163(3):1237-45. PMID: 10415019 Collings, A.,
Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K.,
Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson,
P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1
nef in mice DNA-immunised with non-replicating or self-replicating expression
vectors. Vaccine. 18(5- 6):460-7. Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61 |
| Limitations |
This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
Figure 1: Western Blot testing of HIV-1 recombinant Nef protein. using HIV-1 Nef antibody, clone 3E6 (01-004). Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker.