Antibodies

Mouse monoclonal antibody to HIV-1 Nef

A2-804-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Nef
Immunogen HIV-1 Nef
Immunogen Description

Recombinant Nef protein of HIV-1 B subtype consensus (purified from E. coli)

Uniprot ID -
Alternative Names 3’ORF
Clonality Mouse monoclonal
Clone 3E6
Class IgG1
Reactivity

Reacts with HIV-1 Nef of subtypes A,  B and C; BRU, IIIB, LAV, BH10 strains. Mapped to amino acids 163-173 (SLLHPMSLHGM – B subtypes consensus)

Application ELISA, WB, ICC, RIP
Protocol

ELISA 0.033 to 0.1 µg/ml; WB: 1 µg/ml 

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification

Protein G purification

Buffer PBS pH 7.4, with 0.1% sodium azide.
Unit Size

100 µg

Related Products

HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/antibodies

Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background -
References

Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994. Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Fiala, A.M., R.H. Rhodes, P. Shapshak, I. Nagano, O. Martinez-Maza, A. Diagne, G. Baldwin, and M. Graves. 1996. Regulation of HIV-1 infection in astrocytes: Expression of NEF, TNF-alpha and IL-6 is enhanced in coculture of astrocytes with macrophages. J.Neurovirology, 2, No. 3: 158-166. PMID: 8799208

Koedel, U., Kohleisen B, Sporer B, Lahrtz F, Ovod V, Fontana A, Erfle V, Pfister HW. 1999. HIV type 1 Nef protein is a viral factor for leukocyte recruitment into the central nervous system. J Immunol. 1;163(3):1237-45. PMID: 10415019

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61

Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1:  Western Blot testing of HIV-1 recombinant Nef protein. using HIV-1 Nef antibody, clone 3E6 (01-004). Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker.