Mouse monoclonal antibody to HIV-1 Nef
A2-802-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 Nef |
| Immunogen | HIV-1 Nef |
| Immunogen Description | Recombinant Nef protein of HIV-1 HXB2 (purified from E. coli) |
| Uniprot ID | - |
| Alternative Names | 3’ORF |
| Clonality | Mouse monoclonal |
| Clone | 3D12 |
| Class | IgG1 |
| Reactivity | Reacts with HIV-1 subtypes B and C, BRU, IIIB, and HAN strains. Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HXB2) |
| Application | ELISA, WB, ICC |
| Protocol | ELISA 0.1 to 0.2 µg/ml; WB 2 µg/ml. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20… -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | - |
| References | Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34. Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994. Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481 Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008. Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7. Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61. Maksiutov A.Z. , A.G. Bachinskii, and S.I. Bazhan. 2002 [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459. Lehmann M.H., Walter S, Ylisastigui L, Striebel F, Ovod V, Geyer M, Gluckman JC, Erfle V. 2006 Extracellular HIV-1 Nef increases migration of monocytes. Exp Cell Res. 312 (18):3659-68. |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
Figure 1: Western Blot testing of HIV-1 recombinant Nef protein. using HIV-1 Nef antibody, clone 3D12 (01-002). Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker.