Antibodies

Mouse monoclonal antibody to HIV-1 Nef

A2-807-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Nef
Immunogen HIV-1 Nef
Immunogen Description

Recombinant Nef protein of HIV-1 BRU (purified from E. coli)

Alternative Names 3’ORF
Clonality Mouse monoclonal
Clone 2H12
Class IgG1
Reactivity Reacts with HIV-1 Nef of B and C subtypes, BRU, IIIB, and HAN strains
Epitope: mapped to amino acids 171-190
(-HGMDDPEREVLEWRFDSRLA-) (HIV-1BRU)
Application

ELISA, WB, IHC

Protocol ELISA 0.027 to 0.8 µg/ml; WB 0.8 µg/ml
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size

100 µg

Related Products HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References

Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

 

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994. Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

 

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Koedel, U. , Kohleisen B, Sporer B, Lahrtz F, Ovod V, Fontana A, Erfle V, Pfister HW. 1999 HIV type 1 Nef protein is a viral factor for leukocyte recruitment into the central nervous system. J Immunol. 163(3):1237-45.

Kohleisen, B. , Hutzler P, Shumay E, Ovod V, Erfle V. 2001 HIV-1 Nef co-localizes with the astrocyte-specific cytoskeleton protein GFAP in persistently nef-expressing human astrocytes. J Neurovirol. 7(1):52-5.

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.

Lehmann M.H., Walter S, Ylisastigui L, Striebel F, Ovod V, Geyer M, Gluckman JC, Erfle V. 2006 Extracellular HIV-1 Nef increases migration of monocytes. Exp Cell Res. 312 (18):3659-68

Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western Blot testing of recombinant HIV-1 B subtype Nef protein. using HIV-1 Nef antibody (clone 2H12). Lane 1. Recombinant HIV-1 Nef (B subtype) Lane 2. Protein size marker.