Antibodies

Mouse monoclonal antibody to Hexahistidine (His6) tag

A2-501-100

Datasheet as
Name Mouse monoclonal antibody to Hexahistidine (His6) tag
Immunogen His-tag
Immunogen Description

Purified, recombinant C-terminal His6-tagged protein expressed in E. coli

Clonality Mouse monoclonal
Clone 16-002
Class IgG2b
Reactivity

Tested for detection of C-terminal His6-tag, probably recognizes N-terminal His6-tag

Application ELISA, WB
Protocol ELISA 0.033 to 0.1 µg/ml; WB 0.5 to 2 µg/ml
Purification Protein G purification
Buffer PBS pH 7.4, 0.1% sodium azide
Unit Size 100 µg
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage

Store at -20 or -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western Blot testing of anti His6 monoclonal antobody.

Production of recombinant, C-terminal hexahistidine tag-containing IL-10 (23.2 kDa) was tested from CHO-based cell line supernatant.