Antibodies

Partially humanized recombinant antibody to HCV subtype1b NS5B protein

C2-424-100

Datasheet as
Name Partially humanized recombinant antibody to HCV subtype
1b NS5B protein
Immunogen HCV 1b NS5B
Immunogen Description Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
Uniprot ID Q9WMX2
Alternative Names -
Clonality Partially humanized monoclonal
Clone 9A2
Class IgG1
Reactivity Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)
Application ELISA, WB, IF
Protocol

ELISA titer 1:1000; Western immunoblotting, reduced conditions; dilution 1:1000; Immunofluorescense 1:1000

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Purification

Protein G purification

Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products

This antibody is derived from A2-424-100 mouse monclonal antibody to HCV 1b NS5B, clone 9A2. HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview

References -
Limitations

This product is for research use only

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€299 / 100µg

Figure 1. Western Blot testing of anti-HCV NS5B protein using chimeric anti-HCV NS5B antibody 9A2. Line 1. Protein size marker,  Line 2. 20 ng of purified recombinant HCV NS5B was loaded per lane.

Figure 2.
Immunofluorescence analysis on ETAR protein fused with 9A2 epitope tag. ETAR-9A2 is expressed in CHOEBNALT85 cell line.