Partially humanized recombinant antibody to HCV subtype1b NS5B protein

ELISA titer 1:1000; Western immunoblotting, reduced conditions; dilution 1:1000; Immunofluorescense 1:1000

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Protein G purification

This antibody is derived from A2-424-100 mouse monclonal antibody to HCV 1b NS5B, clone 9A2. HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies

This product is shipped in non-frozen liquid form in ambient conditions

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview

This product is for research use only