Partially humanized recombinant antibody to HCV subtype1b NS5B protein
C2-424-100
Datasheet as| Name | Partially humanized recombinant antibody to HCV subtype 1b NS5B protein |
| Immunogen | HCV 1b NS5B |
| Immunogen Description | Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp) |
| Uniprot ID | Q9WMX2 |
| Alternative Names | - |
| Clonality | Partially humanized monoclonal |
| Clone | 9A2 |
| Class | IgG1 |
| Reactivity | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
| Application | ELISA, WB, IF |
| Protocol |
ELISA titer 1:1000; Western immunoblotting, reduced conditions; dilution 1:1000; Immunofluorescense 1:1000 Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Purification |
Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products |
This antibody is derived from A2-424-100 mouse monclonal antibody to HCV 1b NS5B, clone 9A2. HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping |
This product is shipped in non-frozen liquid form in ambient conditions |
| Storage |
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Background |
Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview |
| References | - |
| Limitations |
This product is for research use only |
Figure 1. Western Blot testing of anti-HCV NS5B protein using chimeric anti-HCV NS5B antibody 9A2. Line 1. Protein size marker, Line 2. 20 ng of purified recombinant HCV NS5B was loaded per lane.
