Partially humanized recombinant antibody to HCV subtype1b NS5B protein
|Name||Partially humanized recombinant antibody to HCV subtype
1b NS5B protein
|Immunogen||HCV 1b NS5B|
|Immunogen Description||Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)|
|Clonality||Partially humanized monoclonal|
|Reactivity||Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)|
|Application||ELISA, WB, IF|
ELISA titer 1:1000; Western immunoblotting, reduced conditions; dilution 1:1000; Immunofluorescense 1:1000
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
Protein G purification
|Buffer||PBS pH 7.4, with 0.1% sodium azide|
|Unit Size||100 µg|
This antibody is derived from A2-424-100 mouse monclonal antibody to HCV 1b NS5B, clone 9A2. HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies
This product is shipped in non-frozen liquid form in ambient conditions
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.
Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview
This product is for research use only
Figure 1. Western Blot testing of anti-HCV NS5B protein using chimeric anti-HCV NS5B antibody 9A2. Line 1. Protein size marker, Line 2. 20 ng of purified recombinant HCV NS5B was loaded per lane.