Antibodies

Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein

A2-428-100

Datasheet as
Name Mouse monoclonal antibody to HCV subtype 1b NS5B
protein
Immunogen HCV 1b NS5B
Immunogen Description Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
Uniprot ID Q9WMX2
Alternative Names -
Clonality Mouse monoclonal
Clone 8G6
Class IgG1
Reactivity Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 77-86 (TVKAKLLSVE)
Application ELISA, WB, IP
Protocol

WB in reduced conditions1 µg/ml

Optimal conditions for IP should be determined for each particular application.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

References
Nikonov A, Juronen E, Ustav M  (2008).  Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
Limitations This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. IP was carried out with NS5B specific mAb 8G6 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (a-NS5B) and a-Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with NS5B specific mAb 10D6



Figure 2. WB analysis of Huh-7 cell line expressing NS5B protein. Detected by 8G6 antibody. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.