Antibodies

Mouse monoclonal antibody to HCV subtype 1b NS5B protein

A2-425-100

Datasheet as
Name Mouse monoclonal antibody to HCV subtype 1b NS5B protein
Immunogen HCV 1b NS5B
Immunogen Description Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
Uniprot ID Q9WMX2
Alternative Names -
Clonality Mouse monoclonal
Clone 10D6
Class IgG1
Reactivity Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)
Application ELISA, WB, IP
Protocol ELISA 0.25 µg/ml; WB in reduced conditions 1 µg/ml.
Optimal conditions for IP should be determined for each particular application.
Optimal conditions for IP should be determined for each particular application
Purification

Protein G purification

Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products

HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies

Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage

Store at -20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

References Nikonov A, Juronen E and Ustav M (2008).  Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
Limitations This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. IP was carried out with NS5B specific mAb 10D6 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (a-NS5B) and a-Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with NS5B specific mAb 10D6


Figure 2. WB analysis of Huh-7 cell line expressing NS5B protein. Detected by 10D6 antibody. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.