Antibodies

Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein

A2-426-100

Datasheet as
Name Mouse monoclonal antibody to HCV subtype 1b NS5B protein
Immunogen HCV 1b NS5B
Immunogen Description

Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Uniprot ID Q9WMX2
Alternative Names -
Clonality Mouse monoclonal
Clone 10D7
Class IgG1
Reactivity Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA)
Application ELISA, WB
Protocol

WB in reduced conditions 1 µg/ml; 

Optimal conditions for IP should be determined for each particular application.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

References Nikonov A, Juronen E and Ustav M (2008).  Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
Limitations This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.