Rabbit polyclonal antibody to human CDNF
300-100
Datasheet as| Name | Rabbit polyclonal antibody to human CDNF |
| Immunogen | Human CDNF |
| Immunogen Description | Recombinant human CDNF protein produced using
CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified
from cell culture supernatant. |
| Uniprot ID | Q49AH0 |
| Alternative Names | ARMETL1 |
| Clonality | Rabbit polyclonal |
| Class | IgG |
| Reactivity | Human, mouse;
others not tested |
| Application | ELISA, WB, IF, IHC-P |
| Protocol |
ELISA 0.1 to 0.2 µg/ml; WB 0.1 to 0.2 µg/ml; IHC-P (on formalin-fixed, paraffin-embedded tissues, antigen retrieval) - 5 to 10 µg/ml; Monoclonal antibody working dilution has to be established practically for each particular antigen and assay format |
| Purification | CDNF-affinity purification |
| Buffer | 50% saturated ammonium sulphate in PBS pH 7.4 |
| Unit Size | 100 µg |
| Related Products | Monoclonal antibodies to human CDNF. For more details www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use |
| Background | CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
| Limitations |
This product is for research use only |
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€299 / 100µg
Figure 1. Western Blot testing of anti-CDNF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.
A
B
Figure
2. Immunohistochemistry testing of anti-CDNF rabbit
polyclonal antibody. Analysis was performed using formalin-fixed
paraffin-embedded human cerebral cortex tissue sections from Alzheimer’s
disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6)
for antigen retrieval. Incubation with primary antibody at 5 µg/ml was
performed overnight at 4°C. DAKO EnVisionTM Detection System,
Peroxidase/DAB was used for visualization. Sections were counterstained with
toluidine blue and mounted with Eukitt mounting medium. A. CDNF staining by rabbit polyclonal anti-CDNF antibody; B. Negative staining without primary
antibody
A
B
Figure
3. Immunofluorescence detection of human
CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF rabbit
polyclonal antibody, dilution 1:3000. Goat ant-rabbit AlexaFluor488 was used as
secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform
(Thermo Scientific) was used for image acquisition (10x objective). Composite
picture was generated using pseudocolors green for CDNF specific signal and
blue for nuclei. A. CDNF-expressing
U2OS cells; B. Negative
control (non-transfected U2OS cells)