Antibodies

Mouse monoclonal antibody to human CDNF

301-100

Datasheet as
Name Mouse monoclonal antibody to human CDNF
Immunogen Human CDNF
Immunogen Description Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant
Uniprot ID Q49AH0
Alternative Names ARMETL1
Clonality Mouse monoclonal
Clone 7D6
Class IgG1
Reactivity Human, mouse; others not tested
Binds to the N-terminal part of the human CDNF (aa 25-125)
Application ELISA, WB, IF
Protocol ELISA (rhCDNF) 0.1 to 0.2 µg/ml; ELISA (rmCDNF) 0.13 to 0.4 µg/ml; WB 0.25 to 1 µg/ml; IF 0.33 to 10 µg/ml; 
Monoclonal antibody working dilution has to be established practically for each particular antigen and assay format  
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to human CDNF. For more details www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20 °C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
References -
Limitations

This product is for research use only

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€299 / 100µg













Figure 1. Western Blot testing of anti-CDNF monoclonal antibody (7D6).  Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium. 

A
B

Figure 2. Immunofluorescence detection of human CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF antibody clone 7D6 at 1 µg/ml.  Goat ant-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for CDNF specific signal and blue for nuclei. A. CDNF-expressing U2OS cells; B. Negative control