Antibodies

Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein

A1-154-100

Datasheet as
Name Mouse monoclonal antibody to Bovine Papillomavirus type 1
E2-protein
Immunogen BPV type 1 E2
Immunogen Description Recombinant full length BPV type 1 E2 protein purified from E. coli
Uniprot ID P03122
Clonality Mouse monoclonal
Clone 5H4
Class IgG
Reactivity Bovine, BPV type 1 E2 protein, mapped to 310-410
Application ELISA, IF
Protocol

ELISA 0.1 to 0.2 µg/ml; IF 0.33 to 20 µg/ml

This is a conformational antibody and does not work in Western immunoblotting analysis or in other denaturative conditions.

Induces DNA/protein complex supershift in mobility shift assay.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Purification

Protein A purification

Buffer PBS with 50% glycerol
Unit Size 100 µg
Related Products 9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
References

Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.

Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25

Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg
A.

B.

Figure 1. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (5H4). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 0.33 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.