Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein
|Name||Mouse monoclonal antibody to Bovine Papillomavirus type 1
|Immunogen||BPV type 1 E2|
|Immunogen Description||Recombinant full length BPV type 1 E2 protein purified from E. coli|
|Reactivity||Bovine, BPV type 1 E2 protein, mapped to 310-410|
ELISA 0.1 to 0.2 µg/ml; IF 0.33 to 20 µg/ml
This is a conformational antibody and does not work in Western immunoblotting analysis or in other denaturative conditions.
Induces DNA/protein complex supershift in mobility shift assay.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
Protein A purification
|Buffer||PBS with 50% glycerol|
|Unit Size||100 µg|
|Related Products||9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies|
|Shipping||This product is shipped in non-frozen liquid form in ambient conditions|
|Storage||Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result|
|Background||E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV|
Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.
Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25
This product is for research use only
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Price per quantity:
€308 / 100µg
€1231 / 500µg
€1999 / 1000µg