Antibodies

Partially humanized, chimeric recombinant monoclonalantibody to Bovine Papillomavirus type 1 E2-protein

C-100-100

Datasheet as
Name Partially humanized, chimeric recombinant monoclonal
antibody to Bovine Papillomavirus type 1 E2-protein
Immunogen BPV type 1 E2
Immunogen Description Recombinant full length BPV type 1 E2 protein purified from E. coli
Uniprot ID P03122
Clonality Partially humanized monoclonal
Clone 5E11
Class IgG1
Reactivity Bovine, BPV type 1 E2 protein amino acids 199-208 (SSTSSDFRDR)
Application WB, IF, IP
Protocol

Western blot: 1/1000 – 1/5000; Immunofluorescence: 1/1000; Immunoprecipitation: Optimal conditions for IP should be determined for each particular application.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification MabSelect purification
Buffer PBS with 50% glycerol
Unit Size 100 µg
Related Products 9 different monoclonal antibodies to BPV type 1 E2 protein. Mouse monoclonal variant of this antibody is A-100-100, clone 5E11 For more information and ordering see full list: www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Background

E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV

Antibody is derived from mouse anti-BPV E2 monoclonal antoibody 5E11 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 5E11 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview

References -
Limitations

This product is for research use only

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€299 / 100µg













Figure 1.
Western Blot testing of anti-BPV E2 monoclonal antibody (5E11). Lane 1. Protein size marker; Lane 2. 5E11-epitope tag-fused MBP expressed in E. coli.


Figure 2. Immunofluorescence analysis of ETAR fused with 5E11-tag sequence. Humanized anti-BPV E2 antibody was used for detection of ETAR protein.