Antibodies

Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein

A1-100-100

Datasheet as
Name Mouse monoclonal antibody to Bovine Papillomavirus type 1
E2-protein
Immunogen BPV type 1 E2
Immunogen Description Recombinant full length BPV type 1 E2 protein purified from E. coli
Uniprot ID P03122
Clonality Mouse monoclonal
Clone 5E11
Class IgG
Reactivity Bovine, BPV type 1 E2 protein amino acids 199-208 (SSTSSDFRDR)
Application ELISA, WB, IF, IP
Protocol

ELISA 0.02 µg/ml; WB 0.2 to 1.2 µg/ml; IF: 0.4 to 24 µg/ml; 

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Purification Protein G purification
Buffer PBS with 50% glycerol
Unit Size 100 µg
Related Products 9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20 or -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
References

Koljak R, Järving I, Kurg R, Boeglin WE, Varvas K, Valmsen K, Ustav M, Brash AB, and Samel N. 2001. The basis of prostaglandin synthesis in coral, J. Biol. Chem., 276: 7033-7040.

Nigul L, Olspert A, Meier M, Paves M, Talpsep T, and Truve E. 2004. New Plant vectors for Protein Tagging with E2 Epitope, Plant Molecular Biology Reporter, 4: 399 – 407.

Mannan AU, Boehm J, Sauter SM, Rauber A, Byrne PC, Neesen J, and Engel W. 2006. Spastin, the most commonly mutated protein in hereditary spastic paraplegia interacts with reticulon 1 an endoplasmic reticulum protein. Neurogenetics 7: 93-103.

Limitations This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg
A

B

Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (5E11). 

A:Testing of purified recombinant BPV E2. Different amount of purified protein was loaded into the gel. Lane 1. 30 ng; Lane 2. 15 ng; Lane 3. 10 ng; Lane 4. 5 ng; Lane 5. 1 ng.

B: Testing of BPV E2 expressed in CHO cell line. Lane 1. Cell lysate of CHO cells. Lane 2. Cell lysate of CHO cells stably expressing BPV E2

A

B


Figure 2. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (5E11). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 1 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.