Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein
A1-153-100
Datasheet as| Name | Mouse monoclonal antibody to Bovine Papillomavirus type 1 E2-protein |
| Immunogen | BPV type 1 E2 |
| Immunogen Description | Recombinant full length BPV type 1 E2 protein purified from E. coli |
| Uniprot ID | P03122 |
| Clonality | Mouse monoclonal |
| Clone | 3H5 |
| Class | IgG |
| Reactivity | Bovine, BPV type 1 E2 protein, mapped to amino acids 310-410 |
| Application | ELISA, IF |
| Protocol |
ELISA - 0.1 to 0.2 µg/ml; IF 0.5 to 20 µg/ml This is a conformational antibody and does not work in WB or other denaturative conditions. This antibody blocks E1-E2 dependent replication origin complex formation, dissociates protein/DNA complex and therefore inhibits viral replication. |
| Purification | Protein A purification |
| Buffer | PBS with 50% glycerol |
| Unit Size | 100 µg |
| Related Products | 9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Background | E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV |
| References |
Kurg
R,
Parik J, Juronen E, Sedman T, Abroi A,
Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2
protein-specific monoclonal antibodies on papillomavirus DNA replication, J
Virol. 73: 4670-77. |
| Limitations |
This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
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Figure 1. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (3H5). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 0.5 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.