Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein
|Name||Mouse monoclonal antibody to Bovine Papillomavirus type 1
|Immunogen||BPV type 1 E2|
|Immunogen Description||Recombinant full length BPV type 1 E2 protein purified from E. coli|
|Reactivity||Bovine, BPV type 1 E2 protein, mapped to amino acids 310-410|
ELISA - 0.1 to 0.2 µg/ml; IF 0.5 to 20 µg/ml
This is a conformational antibody and does not work in WB or other denaturative conditions.
This antibody blocks E1-E2 dependent replication origin complex formation, dissociates protein/DNA complex and therefore inhibits viral replication.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
|Purification||Protein A purification|
|Buffer||PBS with 50% glycerol|
|Unit Size||100 µg|
|Related Products||9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies|
|Shipping||This product is shipped in non-frozen liquid form in ambient conditions|
|Storage||Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.|
|Background||E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV|
Parik J, Juronen E, Sedman T, Abroi A,
Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2
protein-specific monoclonal antibodies on papillomavirus DNA replication, J
Virol. 73: 4670-77.
This product is for research use only
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