Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein
|Name||Mouse monoclonal antibody to Bovine Papillomavirus type 1
|Immunogen||BPV type 1 E2|
|Immunogen Description||Recombinant full length BPV type 1 E2 protein purified from E. coli|
|Reactivity||Bovine, BPV type 1 E2 protein, mapped to amino acids 197-208 (GVSSTSSDFRDR)|
|Application||ELISA, WB, IF
ELISA 0.05 to 0.1 µg/ml; WB 0.33 to 1 µg/ml; IF 1 µg/ml
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
|Purification||Protein A purification|
|Buffer||PBS with 50% glycerol|
|Unit Size||100 µg|
|Related Products||9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies|
|Shipping||This product is shipped in non-frozen liquid form in ambient conditions|
|Storage||Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.|
|Background||E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV|
Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.
Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25
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Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (3F12). 2 ng of purified recombinant E2 protein was loaded.
Figure 2. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (3F12). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 1 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.