Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein
A1-152-100
Datasheet as| Name | Mouse monoclonal antibody to Bovine Papillomavirus type 1 E2-protein |
| Immunogen | BPV type 1 E2 |
| Immunogen Description | Recombinant full length BPV type 1 E2 protein purified from E. coli |
| Uniprot ID | P03122 |
| Clonality | Mouse monoclonal |
| Clone | 3E8 |
| Class | IgG |
| Reactivity | Bovine, BPV type 1 E2 protein, mapped to amino acids 310-410 |
| Application | ELISA, IF |
| Protocol |
ELISA - 0,2 -1 µg/ml; IF 0.33 to 20 µg/ml This is a conformational antibody and does not work in Western immunoblotting analysis or in other denaturative conditions. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Purification | Protein A purification |
| Buffer | PBS with 50% glycerol |
| Unit Size | 100 µg |
| Related Products | 9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Background | E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV |
| References |
Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77. Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25 |
| Limitations |
This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
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Figure 1. Immunofluorescence testing of anti-BPV E2 monovlonal antibody (3E8). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 0.33 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.