Rabbit polyclonal antibody to bovine DNase I
|Name||Rabbit polyclonal antibody to bovine DNase I
|Immunogen||Bovine DNase I|
|Immunogen Description||Recombinant full length bovine DNase I produced using CHO-based Icosagen Cell Factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant.|
0.05 to 0.1 µg/ml; WB 0.1 to 0.2 µg/ml; IF
0.2 to 12 μg/ml
Antibody working titer has to be established practically for each particular antigen and assay format.
|Purification||Bovine DNaseI-affinity purification|
Concentrated ammonium sulphate in PBS pH 7.4
|Unit Size||100 µg|
|Shipping||Shiping in non-frozen liquid form|
|Storage||Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. Do not freeze|
Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments.
product is for research use only.
Please refer any technical questions to firstname.lastname@example.org
Figure 1. Western Blot testing of rabbit polyclonal antibody (400-100) to bovine DNase I. Lane 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Lane 2. Recombinant bovine DNase I expressed into the supernatant of CHO cell culture medium. Antibody concentration 0.1 µg/ml