Antibodies

Rabbit polyclonal antibody to bovine DNase I

400-100

Datasheet as
Name Rabbit polyclonal antibody to bovine DNase I
Immunogen Bovine DNase I
Immunogen Description Recombinant full length bovine DNase I produced using CHO-based Icosagen Cell Factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant. 
Uniprot ID P00639
Alternative Names DNL1
Clonality Rabbit polyclonal
Class IgG
Reactivity Bovine
Application ELISA, WB
Protocol ELISA 0.05 to 0.1 µg/ml; WB 0.1 to 0.2 µg/ml; IF  0.2 to 12 μg/ml
Antibody working titer has to be established practically for each particular antigen and assay format.
Purification Bovine DNaseI-affinity purification
Buffer

Concentrated ammonium sulphate in PBS pH 7.4

Unit Size 100 µg
Shipping Shiping in non-frozen liquid form
Storage Store at +4°C upon receipt. As product is (NH4)2SOprecipitate, mix well by pipetting or vortexing prior use. Do not freeze
Background

Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments.

Limitations This product is for research use only.

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western Blot testing of rabbit polyclonal antibody (400-100) to bovine DNase I. Lane 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Lane 2. Recombinant bovine DNase I expressed into the supernatant of CHO cell culture medium. Antibody concentration 0.1 µg/ml