Rabbit polyclonal antibody to bovine DNase I
400-100
Datasheet as| Name | Rabbit polyclonal antibody to bovine DNase I |
| Immunogen | Bovine DNase I |
| Immunogen Description | Recombinant full length bovine DNase I produced using CHO-based Icosagen Cell Factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant. |
| Uniprot ID | P00639 |
| Alternative Names | DNL1 |
| Clonality | Rabbit polyclonal |
| Class | IgG |
| Reactivity | Bovine |
| Application | ELISA, WB |
| Protocol | ELISA
0.05 to 0.1 µg/ml; WB 0.1 to 0.2 µg/ml; IF
0.2 to 12 μg/ml
Antibody working titer has to be established practically for each particular antigen and assay format. |
| Purification | Bovine DNaseI-affinity purification |
| Buffer |
Concentrated ammonium sulphate in PBS pH 7.4 |
| Unit Size | 100 µg |
| Shipping | Shiping in non-frozen liquid form |
| Storage | Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. Do not freeze |
| Background |
Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments. |
| Limitations | This
product is for research use only. TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure 1. Western Blot testing of rabbit polyclonal antibody (400-100) to bovine DNase I. Lane 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Lane 2. Recombinant bovine DNase I expressed into the supernatant of CHO cell culture medium. Antibody concentration 0.1 µg/ml