Antibodies

Mouse monoclonal antibody to bovine DNase I

405-100

Datasheet as
Name Mouse monoclonal antibody to bovine DNase I
Immunogen Bovine DNase I
Immunogen Description
Recombinant full length bovine DNaseI produced using CHO-based Icosagen Cell Factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant. 
Uniprot ID P00639
Alternative Names DNL1
Clonality Mouse monoclonal
Clone 6D5
Class IgG1
Reactivity Bovine, cross reactivity to Human
Application ELISA, WB
Protocol
ELISA 0.01 – 0.02 µg/ml; WB  0.1 – 0.2 µg/ml; IF  0.33 – 20 µg/ml
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
Purification Protein G purification
Buffer PBS, pH 7.4
Unit Size 100 µg
Related Products Bovine DNase I polyclonal antibody, cat no 400-100
Shipping

Shiping in non-frozen liquid form

Storage Store at -20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Background

Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments.

Limitations This product is for research use only 

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1.
Western Blot testing of anti-bovine DNaseI monoclonal antibody (6D5). 
Lane 1. Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. 10 µl of supernatant was loaded.
Lane 2. PageRuler Prestained Protein Ladder (#SM0671 Fermentas)