P-101-100
MANF, human recombinant
Datasheet as PDF
Figure 1. SDS-PAGE analysis of human MANF visualized by PageBlue protein staining solution (Fermentas). Line 1. Protein size marker (PageRuler Prestained protein ladder, Fermentas); Line 2. 10 µg of MANF.
Figure 2. Western Blot testing of human MANF using anti-MANF polyclonal antibody (Icosagen AS Cat. No 310-100). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant MANF expressed into the supernatant of CHO cell culture medium
Description
Synonyms
Mesencephalic astrocyte-derived neurotrophic
factor – MANF; ARMET; ARP
Uniprot
P55145
Source
Human
MW
Approximately 18.1 kDa, a single non-glycosylated polypeptide
chain containing 163 amino acids
Expression Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Purified by ion-exchange chromatography and
gel-filtration from serum-free CHO growth media
Concentration
1 mg/ml Concentration of the protein is
determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
Buffer
PBS pH 7.4, concentration
Endotoxin Level
Less than 1EU/mg of protein as determined by LAL
method
Biological Properties
Protein is biologically active. Suitable for cell
culture and in vivo testing. Human
recombinant MANF has been tested in rat 6-OHDA model of Parkinson's disease
and shown neureprotection and neurorestoration. MANF has also shown
neuroprotection in animal models of stroke
QC
SDS-PAGE and Western-Blot analysis (Figure 1 and 2); Mass-spectroscopy:
Purified human MANF is characterized as homogenous
material by mass-spectroscopy
Background
MANF is a trophic factor for midbrain dopamine neurons
in vivo. It prevents the 6-OHDA-
induced degeneration of dopamine neurons in rodent models of Parkinson’s
disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered
after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration
of dopamine neurons in substantia nigra pars compacta
Shipping
Shipped in dry-ice
Storage
Store at -70°C upon receipt. Recommended to aliquot
into smaller quantities. Avoid repeated freeze-thaw cycles
Related Products
Polyclonal and monoclonal antibodies against human
MANF For more information please visit: www.icosagen.com/antibodies
