CUSTOM Protein Production SERVICE
QMCF Technology outperforms a number of transient systems boosting the expression level, process speed and convenience, and cost-effectiveness. Using optimized mammalian QMCF cell lines we have produced several types of proteins, such as
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Cytokines
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Growth factors
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Nucleases
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Ion-channels
- Receptors
- Membrane bound proteins
- Viral glycoproteins
Custom Protein Production Services include
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Cloning |
Expression analysis |
Expression optimization |
Scaled-up production |
Purification |
Quality control |
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Codon optimization and synthesis of cDNA (optional)
Cloning of cDNA into QMCF expression plasmids |
Expression in suspension QMCF/CHO cells in small scale Primary detection of expression and quantification Generation of expression cell banks |
Selection of expression vectors, optimization of growth media and conditions, cell lines Detection of expression and quantification Generation of expression cell banks |
Expanding the culture volume up to 20 L Quantitative analysis |
Purification of the product according to its characteristics and application purpose |
Protein purity, concentration, stability and biological activity analysis |
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3-4 weeks gene synthesis; 1 week cloning |
2-3 days |
Min 4-5 weeks |
2-3 weeks |
Min 1 week |
Inquire |
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Price starts from EUR 1500 for cloning and expression analysis |
Inquire |
From EUR 1000 |
From EUR 1000 |
Inquire | |
Capacities:
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CO2 incubators with Kühner shakers
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WAVE Bioreactor 20/50
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Basic filtration system (vacuum bottles with filter holder)
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ÄKTA chromatograph for purification of proteins in small scale
Read more about protein production and QMCF Technology.
Read more about using QMCF Technology for production virus-like particles and membrane proteins.
