Development
of signal pathway activation assay
service specification
Icosagen provides service for generation of signal pathway activation assay generation. Service includes construction of expression vectors, generation of cell population (CHO and/or U2OS based cell lines) that staby expresses target receptor, optimization of receptor internalization or signal transduction assay, generation of expression cell banks and assay validation. The service is suitable for screening of active compounds in early stage of drug development, investigation of signal transduction mechanisms.
Competitive advantages
Fast and efficient way to generate stable population of the cells expressing receptors for investigation of signal transduction, screening of active compounds for early stages of drug development
Usage of CHO- or U2OS based adherent cell lines
Feasible generation of expression cell banks
Assay optimization and validation using Thermo Scientific ArrayScan VTI and appropriate Cellomics software
