A3-701-100

Mouse monoclonal antibody to Hev b1 (Rubber elongation factor, REF)
Datasheet as PDF

Figure 1:  Western Blot testing of natural latex extract using anti-HevB1 antibody (A3-700-100, clone 1) Line 1. 10 µl of 20x diluted natural latex extract were loaded per line. Line 2. Protein size marker
250€
 100µg
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Immunogen
Hev b1
Immunogen Description
Hev b1-MBP fusion protein expressed and purified from E. coli.
Uniprot ID
P15252
Alternative Names
Rubber elongation factor protein – REF (Hevea brasiliensis)
Clonality
Mouse monoclonal
Clone
1
Class
IgG2a
Reactivity
Recombinant and native Hev b1
Application
ELISA, WB
Protocol

ELISA 1:32 000. Reacts as detection antibody in capture ELISA with Hev b1 binding monoclonal antibody A3-700-100. WB 1:2000 to 1:4000

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification
Protein G affinity purified
Buffer
PBS, 0.1% sodium azide
Concentration
1 mg/ml
Unit Size
100 µg
Related Products

A3-701-100 - mouse monoclonal antibody to Hev b1, used as a binding antibody in pair with detection antibody A3-700-100 in capture ELISA.

Monoclonal antibodies to Hev b3, Hev b5 and Hev b6.02 are available. For more information see www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background
Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02.
Refrences

ASTM D7427 - 08e1 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived from Latex.

Koh D, Ng V, Leow YH, Goh CL. (2005) A study of natural rubber latex allergens in gloves used by healthcare workers in Singapore. Br J Dermatol. 153(5):954-9.

Palosuo T, Alenius H, Turjanmaa K. (2002) Quantitation of latex allergens. Methods. 27(1):52-8.

Peixinho C, Tavares-Ratado P, Tomás MR, Taborda-Barata L, Tomaz CT. (2008) Latex allergy: new insights to explain different sensitization profiles in different risk groups. Br J Dermatol. 159(1):132-6.

Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C, Meyer DM. (2007) The immuno cross-reactivity of gutta percha points. Dent Mater. 23(3):380-4
Limitations
This product is for research use only