Figure 1: Western Blot testing of natural latex extract using anti-HevB1 antibody (A3-700-100, clone 1) Line 1. 10 µl of 20x diluted natural latex extract were loaded per line. Line 2. Protein size marker
ELISA 1:32 000. Reacts as detection antibody in capture ELISA with Hev b1 binding monoclonal antibody A3-700-100. WB 1:2000 to 1:4000Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
A3-701-100 - mouse monoclonal antibody to Hev b1, used as a binding antibody in pair with detection antibody A3-700-100 in capture ELISA.Monoclonal antibodies to Hev b3, Hev b5 and Hev b6.02 are available. For more information see www.icosagen.com/antibodies
ASTM D7427 - 08e1 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived from Latex.
Koh D, Ng V, Leow YH, Goh CL. (2005) A study of natural rubber latex allergens in gloves used by healthcare workers in Singapore. Br J Dermatol. 153(5):954-9.
Palosuo T, Alenius H, Turjanmaa K. (2002) Quantitation of latex allergens. Methods. 27(1):52-8.
Peixinho C, Tavares-Ratado P, Tomás MR, Taborda-Barata L, Tomaz CT. (2008) Latex allergy: new insights to explain different sensitization profiles in different risk groups. Br J Dermatol. 159(1):132-6.Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C, Meyer DM. (2007) The immuno cross-reactivity of gutta percha points. Dent Mater. 23(3):380-4