A1-910-100
Rabbit polyclonal antibody to MLV p30
Datasheet as PDF
Figure 1. Western Blot testing of MLV-gag antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 2. HEK293 cells; Line 3. MLV-gag protein expressed in HEK293 cells; Line 4. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 5. CHO cells; Line 6. MLV-gag protein expressed in CHO cells. 10 000 cells were lysed and loaded into each lane.
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Immunogen
MLV p30
Immunogen Description
Full length recombinant MLV-Gag p30 capside protein,
purified from E. coli
Uniprot ID
-
Alternative Names
-
Clonality
Rabbit polyclonal
Clone
-
Class
IgG
Reactivity
MLV Gag, p30
Application
ELISA, WB
Protocol
WB 1:1500
Polyclonal antibody working titer has to be established practically for each particular antigen and assay formatPurification
MLV p30 affinity purified
Buffer
Concentrated ammonium sulphide in PBS pH 7.4.
Concentration
1 mg/ml
Unit Size
100 µg
Related Products
Monoclonal and polyclonal antibodies to MLV p30. For more information visit www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in
ambient conditions
Storage
Store at +4°C upon receipt. As product is (NH4)2SO4
precipitate, mix well by pipetting or vortexing prior use
Background
Murine leukemia viruses belong to family of
Retroviruses group and are named for their ability to cause cancer in mice.
Some form of MLV’s are used in cancer research. The retroviral Gag protein plays important
roles at several stages in the viral life cycle. Late in infection, the Gag
precursor protein acts to mediate the assembly and release of virion particles.
After
budding, the core matures by cleavage of Gag precursor protein into
the mature core proteins, the matrix (MA), capsid (CA) and
nucleocapsid proteins. These separated Gag proteins then play roles in the early stages of
infection
Refrences
-
Limitations
This
product is for research use only
