Rabbit polyclonal antibody to MLV p30
Figure 1. Western
Blot testing of MLV-gag antibody. Line
1. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas);
Line 2. HEK293 cells; Line 3. MLV-gag protein expressed in
HEK293 cells; Line 4. PageRuler
Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 5. CHO cells; Line 6. MLV-gag protein expressed in CHO cells. 10 000 cells
were lysed and loaded into each lane.
Full length recombinant MLV-Gag p30 capside protein,
purified from E. coli
Polyclonal antibody working titer has to be established practically for each particular
antigen and assay format
MLV p30 affinity purified
Concentrated ammonium sulphide in PBS pH 7.4.
This product is shipped in non-frozen liquid form in
Store at +4°C upon receipt. As product is (NH4)2SO4
precipitate, mix well by pipetting or vortexing prior use
Murine leukemia viruses belong to family of
Retroviruses group and are named for their ability to cause cancer in mice.
Some form of MLV’s are used in cancer research. The retroviral Gag protein plays important
roles at several stages in the viral life cycle. Late in infection, the Gag
precursor protein acts to mediate the assembly and release of virion particles.
budding, the core matures by cleavage of Gag precursor protein into
the mature core proteins, the matrix (MA), capsid (CA) and
nucleocapsid proteins. These separated Gag proteins then play roles in the early stages of
product is for research use only