310-100
Rabbit polyclonal antibody to human MANF
(Mesencephalic astrocyte-derived neurotrophic factor)
(Mesencephalic astrocyte-derived neurotrophic factor)
Datasheet as PDF
Figure 1. Western Blot testing of anti-MANF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85 cell line
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Immunogen
Human MANF
Immunogen Description
Recombinant human MANF protein produced using CHO-based
Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified
from cell culture supernatant
Uniprot ID
P55145
Alternative Names
ARMET, ARP
Clonality
Rabbit polyclonal
Clone
-
Class
IgG
Reactivity
Human MANF
Application
ELISA, WB
Protocol
ELISA 1:200 000; Western immunoblotting 1:2000 – 1:6000, IHC, IF
No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Polyclonal antibody working titer has to be established practically for each particular antigen and assay formatPurification
MANF-affinity purified
Buffer
Concentrated ammonium sulphate in PBS pH 7.4.
Concentration
0.45 mg/ml
Unit Size
100 µg
Related Products
Monoclonal and polyclonal antibodies to human MANF. For more information visit www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in
ambient conditions
Storage
Storage:
Store
at +4°C upon receipt. As product is (NH4)2SO4
precipitate, mix well by pipetting or vortexing prior use
Background
MANF is a trophic factor for midbrain dopamine neurons
in vivo. It prevents the 6-OHDA-
induced degeneration of dopamine neurons in rodent models of Parkinson’s
disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered
after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration
of dopamine neurons in substantia nigra pars compacta
Refrences
-
Limitations
This
product is for research use only
