A2-604-100
Mouse monoclonal antibody to human laminin a4
Datasheet as PDF
Figure 1. Reactivity of laminin α4 chain specific monoclonal antibody 6C3 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions).
€250 100µg
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Immunogen
Human laminin a4
Immunogen Description
Immunoaffinity-purified Laminin 411 (laminin-8
(α4β1γ1) from platelets
Uniprot ID
Q16363
Alternative Names
-
Clonality
Mouse monoclonal
Clone
6C3
Class
IgG1
Reactivity
Human laminin α4
Application
ELISA, WB, IP
Protocol
ELISA titer 1:4000; Western immunoblotting, reduced conditions; dilution 1:1 000 - 1:2 000; Immunoprecipitation; dilution 1:20
Monoclonal antibody working titer has to be established practically for each particular antigen and assay formatPurification
IgG fraction purified
with Protein G affinity chromatorgaphy
Buffer
PBS pH 7.4, with 0.1% sodium azide
Concentration
1 mg/ml
Unit Size
100 µg
Related Products
Monoclonal antibodies to human laminin. For more information visit www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in
ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into
aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
Laminins are a family of large heterotrimeric proteins
that promote cell adhesion and migration via integrins and other cell-surface
receptors. They are major components of basement membranes of blood vessels and
other tissue compartments, and are synthesized by numerous cell types. Through
combination,laminin chains constitute 15 laminin isoforms. Laminin isoforms,
particularly their α chain, are expressed in a cell- and tissue-specific
manner, and are differentially recognized by integrins
Refrences
Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. (2004) An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66.
Kawataki T, Yamane T, Naganuma H, Rousselle P, Andurén I, Tryggvason K, Patarroyo M. (2007)
Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. Exp Cell ResLimitations
This
product is for research use only
