Figure 1. Western-Blot analysis of HPV-18 E2 protein. WB was was carried out with 2E7 Mab using the lysates of U20S and Hela cells. Lanes 1 and 4 are according cells transfected with plasmid expressing HPV-18  E2 protein, lanes 2 and 3 are   plain U2OS and Hela cells. Last lane represents marker.

Figure 2.  IF analysis of HPV-18 E2 protein in U2OS cells.IF was carried out with Hela cells transfected with plasmid expressing HPV-18 E2 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-18 E2 detected by Mab 2E7