A2-501-100
Mouse monoclonal antibody to Hexahistidine (His6) tag
Datasheet as PDF
Figure 1. Western Blot testing of anti His6 monoclonal antobody. Production of recombinant, C-terminal hexahistidine tag-containing IL-10 (23.2 kDa) was tested from CHO-based cell line supernatant.
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Immunogen
His-tag
Immunogen Description
Purified, recombinant C-terminal His6-tagged protein expressed in E. coli
Uniprot ID
-
Alternative Names
-
Clonality
Mouse monoclonal
Clone
16-002
Class
IgG2b
Reactivity
Tested for detection of C-terminal His6-tag, probably recognizes N-terminal His6-tag
Application
ELISA, WB
Protocol
Western immunoblotting, recommended dilution 1:500-1:2000
Purification
purified by Protein G affinity chromatography
Buffer
PBS pH7.4, with 15mM sodium azide
Concentration
1 mg/ml
Unit Size
100 µg
Related Products
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Shipping
This product is shipped in non-frozen liquid form in
ambient conditions
Storage
Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Background
-
Refrences
-
Limitations
This product is for research use only
