A2-426-100

Mouse monoclonal antibody to HCV subtype 1b NS5B
protein
Datasheet as PDF

Figure 1. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.



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Immunogen
HCV 1b NS5B
Immunogen Description

Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Uniprot ID
Q9WMX2
Alternative Names
-
Clonality
Mouse monoclonal
Clone
10D7
Class
IgG1
Reactivity
Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA)
Application
ELISA, WB
Protocol

ELISA titer 1:1000; Western immunoblotting, reduced conditions; dilution 1:1000

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification
purified by protein G affinity chromatography
Buffer
PBS pH 7.4, with 0.1% sodium azide
Concentration
1 mg/ml
Unit Size
100 µg
Related Products
HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

Refrences
Andrei Nikonov, Erkki Juronen and Mart Ustav (2008).  Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
Limitations

This product is for research use only