A-101-100

Mouse monoclonal antibody to Bovine Papillomavirus type 1
E2-protein
Datasheet as PDF


Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (3F12). Testing of purified recombinant E2. 2 ng of purified protein was loaded into the gel.


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Immunogen
BPV type 1 E2
Immunogen Description
Recombinant full length BPV type 1 E2 protein purified from E. coli
Uniprot ID
P03122
Alternative Names
-
Clonality
Mouse monoclonal
Clone
3F12
Class
IgG
Reactivity
Bovine, BPV type 1 E2 protein, mapped to amino acids 197-208 (GVSSTSSDFRDR)
Application
WB, IF
Protocol

Western blot:  1/1000 – 1/3000; Immunofluorescence:   1/1000

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Purification
Protein A affinity chromatography
Buffer
PBS with 50% glycerol
Concentration
1 mg/ml
Unit Size
100 µg
Related Products
9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Background
E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
Refrences

Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.

 Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25

Limitations
This product is for research use only