Mouse monoclonal antibody to Bovine Papillomavirus type 1
Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (1E4). Testing of purified recombinant E2.
Different amount of purified protein was loaded into the gel. Line 1. 15 ng;
Line 2. 10 ng; Line 3. 5 ng; Line 4. 1
Recombinant full length BPV type 1 E2 protein purified
from E. coli
Bovine, BPV type 1 E2 protein, mapped
to amino acids 250-280
Western blot: 1/1000 – 1/3000; Immunofluorescence: 1/1000
antibody working titer has to be established practically for each particular antigen
and assay format.
Protein A affinity chromatography
PBS with 50% glycerol
9 different monoclonal antibodies to BPV type 1 E2
protein. For more information and ordering see full list:
This product is shipped in non-frozen liquid form in
Store at – 20 or -70 °C upon receipt. Divide antibody
into aliquots prior usage. Avoid multiple freeze-thaw cycles as product
degradation may result.
E2 protein plays a central role in the viral life
cycle as it regulates both transcription and replication of the viral genome.
Can either activate or repress transcription. The E1-E2 protein complex binds to
the origin of DNA replication activating replication of the BPV
Parik J, Juronen E, Sedman T, Abroi A,
Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2
protein-specific monoclonal antibodies on papillomavirus DNA replication, J
Virol. 73: 4670-77.
Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine
papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22):
product is for research use only