Figure 1. Reactivity of laminin α4 chain specific monoclonal antibody 3H2 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions) and on human embryonic kidney preparation
Human laminin α4
ELISA titer 1:64 000; Western immunoblotting, reduced conditions; dilution 1:1 000 - 1:2 000; Immunoprecipitation; dilution 1:20; Immunohistochemistry; dilution 1:100 -1:200
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination,laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their α chain, are expressed in a cell- and tissue-specific manner, and are differentiallyrecognized by integrins
Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. (2004) An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66.
Kawataki T, Yamane T, Naganuma H, Rousselle P, Andurén I, Tryggvason K, Patarroyo M. (2007)Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. Exp Cell Res